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Encompassing Amino Acids: 596-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 ?g /ml recombinant EPHA2.
Description: Human EPHA2 (GenBank Accession No. NM_004431), a.a. 596-end with N-terminal His-tag, MW=47 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.
Genbank: NM_004431
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: P29317
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nakada,M., et al., Cancer Res. 64 (9), 3179-3185 (2004).
2. Kataoka,H.,et al., J. Cancer Res. Clin. Oncol. 128 (7), 343-348 (2002).