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Encompassing Amino Acids: 571-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: EPHA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases that bind the ephrin-A ligand and have diverse cellular function. Analysis of human colorectal, breast, lung and pancreatic cancer samples shows somatic mutations in the EPHA3 gene (1). EPHA3 gene expression can be regulated by CD28 and IGF-1 in Jurkat cells and expression of EPHA3 is associated with adherence and motility of malignant T cells (2).
Description: Recombinant human EPHA3 (a.a. 571-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: BC063282
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q6P4R6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wood, L D. et al: Somatic mutations of GUCY2F, EPHA3, and NTRK3 in human cancers. Hum Mutat. 2006 Oct;27(10):1060-1.
2. Smith, L M. et al: EphA3 is induced by CD28 and IGF-1 and regulates cell adhesion. Exp Cell Res. 2004 Jan 15;292(2):295-303.
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