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Encompassing Amino Acids: 563-987
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: ADP-Glo assay with 0.2 mg/ml poly-(Glu4:Tyr) as a substrate and 100 µM ATP at final concentration. Reaction was done for 50 minutes at 30°C. ADP-Glo for 45 minutes at RT, and Kinase detection for 55 minutes at RT.
Description: Human EPHB4 (GenBank Accession No. NM_004444), a.a. 563-987 with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_004444
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: P54760
Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bruhl,T., et al., Circ. Res. 94 (6), 743-751 (2004).
2. Fuller,T., et al., J. Cell. Sci. 116 (Pt 12), 2461-2470 (2003).
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