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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate ERK2 with 0.25 mg/ml Myelin basic Protein (substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
Description: Human ERK2, also known as MAPK1 or MAPKp42, GenBank Accession No. NM_002745, full length, with N-terminal GST-tag, MW ~68 kDa, expressed in E. coli and activated by MEK1 in vitro.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Genbank: NM_002745
Purity: ≥90%
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P28482
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Girault, J.A., et al. Curr Opin Pharmacol. Feb,7(1):77-85 (2007)
2. Fyffe-Maricich SL., et al. J. Neurosci. Jan 19,31(3):843-50 (2011).
3. Ossum, C.G., et al. Cell Physiol Biochem. 27(2):109-20 (2011).
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