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Encompassing Amino Acids: 468-977 (end)
Applications: Useful for studying enzyme kinetics and screening small molecule inhibitors.
Assay Conditions: 10 µl of varying amounts of ERN1 was mixed with 5 µl of 1 mg/ml myelin basic protein substrate and 5 µl of H2O. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
Description: Human endoplasmic reticulumto-nucleus signaling 1 (ERN1), also known asinositol-requiring protein 1 (IRE1), GenBankAccession No. NM_001433, a.a. 468-977(end) MW = 87 kDa, expressed in abaculovirus-infected Sf9 insect cellexpression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Genbank: No. NM_001433
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O75460
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Niwa, M., et al., Cell. 1999; 99: 691-702.2. Iwawaki, T. et al., Proc. Nat. Acad. Sci. 2009; 106: 16657-16662.
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