Target: ERp57
Conjugate: Unconjugated
Product Type: Monoclonal
Clone Number: 4F9
Immunogen: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
Swiss-Prot: P30101
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~57 kDa protein.
Cellular Localization: Endoplasmic Reticulum,Endoplasmic Reticulum Lumen,Melanosome
Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
References: 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.2. Williams D.B. (2005) J Cell Sci. 119: 615-6233. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.5. Oliver J.D., et al. (1997) Science 275: 86-88.6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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