Conjugate: PE.
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Recommended Usage: This kit is intended for RUO in the purification and identification of human exosomes from human urine samples. For flow cytometric detection, (greater than)0,125 μg which corresponds with (greater than)1,5 * 108 vesicles form PC3 cell culture media.
Description: Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping. 1. Yáñez-Mó M, Siljander P, Andreu Z, Bedina Zavec A, Borrás F, Buzas E et al. Biological properties of extracellular vesicles and their physiological functions. Journal of Extracellular Vesicles. 2015;4(1):27066.
This kit is intended for RUO in the purification and identification of human exosomes from human urine samples. For flow cytometric detection, >0,125 μg which corresponds with >1,5 * 108 vesicles form PC3 cell culture media.
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