Notes: Cayman’s Extracellular Vesicle Isolation Kit (Tissue Culture) provides a simple, quick, and convenient precipitation-based method for isolating extracellular vesicles from tissue culture media. The volume of Extracellular Vesicle Isolation Reagent provided is sufficient for processing up to 40 ml of media. Also provided is a storage medium for stabilizing extracellular vesicles during frozen storage. This optional reagent will not interfere with protein assays and is formulated to be compatible with typical downstream analyses.