Formulation: 1 mM Sodium citrate, pH 6.4
Shelf life (days): 180
Notes: EZ Cap™ EGFP mRNA encodes for enhanced GFP (EGFP), a variant of A. victoria GFP that contains chromophore mutations that increase the sensitivity of the protein and displays excitation and emission maxima of 489 and 511 nm, respectively.{72072} It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap™ EGFP mRNA is also polyadenylated and modified with 5-methoxy-UTP (mo5U) to inhibit RNA-mediated innate immune activation. Encapsulation of EZ Cap™ EGFP mRNA (mo5U) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of EGFP in vitro or in vivo.{51488,51489}