Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Description: The EZH2 (Y641N) Chemiluminescent Assay Kit is designed tomeasure activity of the mutant EZH2 complex (EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to theEZH2 (Y641N) Assay Kit is a highly specific antibody that recognizes methylatedHistone H3K27. With this kit, only three simple steps on a microtiter plate are requiredfor methyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the strips are treated with an HRP-labeled secondaryantibody followed by addition of the HRP substrate to produce chemiluminescence thatcan then be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with 8-well strips precoated with histone H3
Uniprot: Q15910
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dillon, S.C., et al. Genome Biology 2005; 6:227.2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.
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