Abbreviation: F-TESTO
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum, plasma.
Tested Sample Types: serum, plasma
Assay Time: 2.5h
Sensitivity: 18.75 pg/mL
Detection Range: 31.25-2000 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal F-TESTO. During the reaction, Universal F-TESTO in the sample or standard competes with a fixed amount of Universal F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal F-TESTO in samples.No significant cross-reactivity or interference between Universal F-TESTO and analogues was observed
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