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Encompassing Amino Acids: 411-686
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30µg/ml Poly (Glu:Tyr)4:1 substrate, and 1 µg/ml recombinant FAK.
Description: Human FAK (GenBank Accession No. L13616), a.a. 411-686 with N-terminal GST-tag, MW=62 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: L13616
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q05397
Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lim, ST. et al., Mol Cell. 2008 Jan 18,29(1):9-22.
2. Gabarra-Niecko, V. et al., Cancer Metastasis Rev. 2003 Dec,22(4):359-74.
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