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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Fibroblast Activation Protein, or FAP, is a type II membrane serine protease of the dipeptidyl peptidase (DPP) subfamily. It is involved in wound healing and tissue repair. FAP upregulation is seen on activated stromal fibroblasts present in cancer, including breast, lung, prostate, pancreatic and cervical cancer. It has an impact on the tumor microenvironment (TME), where it reduces the levels of PEDF (pigment epithelium derived factor), angiopoietin-1 and VEGF-c (vascular endothelial growth factor c) and increase TGF-β (transforming growth factor β) and create an immunosuppressive environment. It is used as biomarker for pro-tumorigenic stroma and links to a poor prognosis. Studies in animal models have shown that knock-out of FAP reduces the metastasis of pancreatic ductal adenocarcinomas (PDA). It has thus been proposed as a candidate target for small molecule inhibitors for cancer therapy applications. Additionally, its expression profile makes it a good candidate for CAR-T applications, with pre-clinical and clinical trials currently ongoing.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The FAP Fluorogenic Assay Kit is designed to measure FAP (fibroblast activation protein) protease activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant FAP enzyme (amino acids 29-760), DPP substrate, and DPP assay buffer for 100 enzyme reactions.The DPP fluorogenic substrate is incubated with a sample containing FAP enzyme to produce a fluorophore that can then be measured using a fluorescence reader.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Supplied in a convenient 96-well format, with purified FAP enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.
Uniprot: Q12884
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Pure E. and Blomberg R., 2018, Oncogene 37: 4343-4357.Bughda R., et al., 2021, Immunotargets Ther. 10:313-323.
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