Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The FBXL10 Chemiluminescent Assay Kit is designed to measure FBXL10activity for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, andCXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyllysine4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate.With this kit, only three simple steps on a microtiter plate are required for detection ofdemethylase activity. First, FBXL10 enzyme is incubated with the methylated H3 peptide for onehour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondaryantibody followed by the addition of the HRP substrate to produce chemiluminescence that can bemeasured using a chemiluminescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: The FBXL10 Chemiluminescent Assay Kit comes in a convenient 96-well strip plate format, precoated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified FBXL10 for 100 enzyme
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Iwase, S., et al. Cell 2007; 128(6):1077-1088.
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