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Encompassing Amino Acids: 399-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate: 2 µM Tyr peptide 4 from Invitrogen.
Description: Human FGFR2 (GenBank Accession No. NM_000141), a.a. 399-end with N-terminal GST-tag, MW= 74 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Genbank: NM_000141
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P21802
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lew, E.D. et al., Proc. Natl. Acad. Sci. U.S.A. 104 (50), 19802-19807 (2007).
2. Pollock , PM. et al., Oncogene. 26(50):7158-62 (2007).
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