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Encompassing Amino Acids: 447-761
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using 0.2 mg/mL Poly-(Glu:Tyr) substrate and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human Fibroblast growth factor receptor 3 (FGFR3), also known as JTK4, CD333, CEK2 and ACH, GenBank Accession No. NM_00142, a.a. 447-761, with N-terminal His-Avi-tag, expressed in an Sf9 infected baculovirus expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW = 39 kDa. This protein was isolated from a co-expression with PTP1B. Percentage of phosphorylation ≤5%.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Genbank: NM_00142
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-Avi-tag
Uniprot: P22607
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cappellen, D. et al., Nature Genetics. (1999); 23:18-20.
2. Bellus, G.A. et al., AM. J. Hum. Genet. (1995); 56(2):368-373.
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