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Encompassing Amino Acids: 460-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Description: Human FGFR4 (GenBank Accession No. NM_002011), a.a. 460-end with N-terminal GST-tag, MW= 65 kDa, expressed in Sf9 cells via a baculovirus expression system
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Genbank: NM_002011
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P22455
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Holtrich, U. et al., Proc. Nat. Acad. Sci. (1991), 88:10411-10415.
2. Roidl, A. et al., Clin Cancer Res. (2009 Mar 15), 15(6):2058-66.
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