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Applications: 1. In vitro and in vivo Bioluminescence Imaging
2. Use as a negative control in CAR-T or NK co-culture killing assays
Background: The K562 cell line was established from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The Firefly Luciferase K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target for the natural killer assay. The signal generated by the firefly luciferase reporter is proportional to cell numbers.
Description: Recombinant K562 cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Thaw Medium 2 (BPS Bioscience #60184):
RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 2D (BPS Bioscience #79639):
RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 µg/ml of Hygromycin B.
Host Cell Line: K562, a human myelogenous leukemia cell line derived from a patient with CML; suspension.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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