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Applications: 1. Use as an internal control in CAR-T or NK co-culture killing assays
2. In vitro and in vivo Bioluminescence Imaging
Background: The Raji line was established from a Burkitt's lymphoma patient. Raji cells constitutively express B cell antigens CD19, CD20, and CD22, and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies such as Chimeric Antigen Receptor (CAR) T-cells. The Firefly Luciferase - Raji Recombinant Cell Line makes an excellent target for CAR-T or NK cells targeting CD19, CD20, or CD22. The signal generated by the firefly luciferase reporter is proportional to Raji cell numbers and facilitates the quantification of Raji cells killing upon co-culture with CAR-T or NK cells.
Description: Recombinant Raji cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.
Formulation: Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 2D (BPS Bioscience #79639):
RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 µg/ml of Hygromycin B.
Host Cell Line: Raji, a human B lymphoblastoid cell line derived from a patient with Burkitt's lymphoma, suspension
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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