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Applications: Use as a control for co-culture killing assays.
Background: RPMI 8226 cells are human B cells isolated from a plasmacytoma/myeloma patient. RPMI 8226 cells constitutively express B-Cell Maturation Antigen (BCMA), and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies, such as Chimeric Antigen Receptor (CAR) T cells. The Firefly Luciferase - RPMI 8226 Recombinant Cell Line makes an excellent target to measure specific killing of CAR-T or NK cells targeting BCMA.
Description: Recombinant RPMI 8226 cells constitutively expressing firefly (Photinus pyralis) luciferase.
Genbank: BAA93575.1
Host Cell Line: RPMI 8226
Mycoplasma Testing: This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains 2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: BSL-1
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