Target: FKBP51
Conjugate: Biotin
Product Type: Monoclonal
Clone Number: Hi51B
Immunogen: Synthetic peptide corresponding to the residues of human FKBP51
Swiss-Prot: Q13451
Purification: Protein G Purified
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~51kDa.
Cellular Localization: Cytoplasm,Nucleus
Scientific Background: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3).The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3).FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
References: 1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394.2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600.3. Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353.4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201.5. Hubler T.R. et al. (2003) Endocrinology 144(6): 2380- 2387.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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