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Encompassing Amino Acids: 564-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate : 2 µM Tyr peptide 2 from Invitrogen.
Description: Human Flt3 (GenBank Accession No. NM_004119), a.a. 564-end with N-terminal His-tag, MW=55 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
Genbank: NM_004119
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P36888
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Takahashi,S., et al., Leuk. Res. 29 (8), 893-899 (2005).
2. Levis,M., et al., Blood 106 (2), 673-680 (2005).
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