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Applications: Great for studying enzyme kinetics and HTS applications.
Background: The methylation of internal adenosines at the N6 position (m6A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer's disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m6A demethylase identified.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The FTO Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate precoated with methylated FTO substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified FTO for 96 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zhou Y., et al. J Biomed Sci. 2017; 24(1):65.
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