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Encompassing Amino Acids: 32-505
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human FTO, also known as Fat mass and obesity-associated protein, ALKBH9; BMIQ14; GDFD; KIAA1752, and alpha-ketoglutarate-dependent dioxygenase, GenBank Accession No. NM_001080432, a.a 32-505 (end), with N-terminal His-Tag and expressed in a E. coli cell expression system. MW = 56 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Genbank: NM_001080432
Storage Stability: At least 6 months at -80°C.
Tags: N-Terminal His-Tag
Uniprot: Q9C0B1
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Frayling, T., et al., Science. 2007; 316(5826): 889-894.
2. Dina, C., et al., Nature Genetics. 2007; 39(6): 724-726.
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