Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Background: Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease. This protease is thought to cleave and activate more than 150 mammalian, viral and bacterial substrates. Among them are viral envelope glycoproteins and bacterial toxins, as well as cellular factors that can promote tumor development and growth. Cleavage of the SARS coronavirus spike glycoprotein by Furin is a critical step in viral fusion and entry, making furin a potential target for SARS-CoV-2 therapy.
Description: The Furin Protease Assay Kit is designed to measure Furin Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
Storage Stability: At least one year from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient 96-well format, with purified Furin, fluorogenic substrate, and Furin assay buffer for 100 enzyme reactions. The Furin inhibitor Chloromethylketone is also included as a positive control.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Braun, E. and Sauter, D. (2019). Furin-mediated protein processing in infectious diseases and cancer. Clin Transl Immunol, 8: e1073. doi:10.1002/cti2.1073.
2. Follis, K.E., et al. (2006). Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology. 350(2):358-369. doi:10.1016/j.virol.2006.02.003
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