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Applications: Test antibodies against CD15 or FUT4.Screen inhibitors or neutralizing antibodies which disrupt the interaction between CD15 and partner proteins/ligands on other cells.
Background: FUT4 (fucosyltransferase 4) is an enzyme that attaches fucose to N-acetyllactosamine polysaccharides during the glycosylation process. It is directly involved in the synthesis of the non-sialylated antigen, Lewis x (also known as cluster of differentiation CD15, or SSEA-1), which is attached to O-glycans on the cell surface and plays a role in cell-cell interactions. CD15 is one of the most important blood group antigens and is expressed in several types of immune cells. It functions in leukocyte homing and sperm-egg recognition. It has been implicated in human diseases such as leukocyte adhesion deficiency type 2 and several types of blood cancer, and is believed to play a role in cancer metastasis.
Description: Recombinant clonal CHO cell line stably expressing full length human FUT4 (fucosyltransferase 4). Since FUT4 controls the synthesis of antigen CD15, forced expression of FUT4 results in overexpression of CD15 at the cell surface, which was confirmed by flow cytometry. The stable clonal cell line was selected for high levels of CD15 expression compared to the parental CHO-K1 cell line.
Host Cell Line: CHO
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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