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Encompassing Amino Acids: 2-537(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 min. at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Description: Human FYN (GenBank Accession No. NM_002037), a.a 2-537(end) with N-terminal FLAG-tag, MW= 61 kDa, expressed in Sf9 insect cells.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, and 10% glycerol
Genbank: NM_002037
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P06241
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ban, K. et al., Blood, 111 (5), 2409-8 (2008)
2. Hoe, HS. et al., J.Biol.Chem 283 (10), 6288-99 (2008)
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