THUNDER GAPDH TR-FRET Cell Signaling Assay kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total GAPDH in cell lysates.

Components: Eu-Total GAPDH (5 µL), Acc-Total GAPDH (20 µL), GAPDH control lysate (100 µL), 5X Lysis Buffer 3 (1 mL), 10X TR-FRET Detection buffer (50 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Total GAPDH.

Assay Target Class: Housekeeping protein (loading control).

SwissProt: P04406.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-GAPDH-100
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-GAPDH-100
• Package Unit: 100 Points
• Quantity Unit: PAK
• Reactivity: Human, Mouse (Murine)
• Application: Cell-based Assay
• Human Gene ID: 2597