Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total GAPDH in cell lysates.
Components: Eu-Total GAPDH (25 µL), Acc-Total GAPDH (100 µL), GAPDH control lysate (500 µL), 5X Lysis Buffer 3 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Total GAPDH.
Assay Target Class: Housekeeping protein (loading control).
SwissProt: P04406.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).