Application Note: WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Positive Control: HeLa expressing GFP-tagged protein
Form: Liquid
Buffer (with preservative): TBS, 1% BSA, 0.005% Thimerosal.
Concentration: 0.74 mg/ml (Please refer to the vial label for the specific concentration.)
Background: The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.
Immunogen: Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of GFP. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: HRP
Full Name: Green Fluorescent Protein