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Applications: Monitor Hedgehog signaling pathway activity.
Screen for activators or inhibitors of the Hedgehog signaling pathway.
Background: The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of human cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor "Patched" (PTCH). This binding releases PTCH inhibition of Smoothened (Smo) and allows Smo to activate the Gli family of transcription factors, leading to transcription and expression of hedgehog signal target genes.
Biological Activity: This cell line is validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway.
Description: The Gli Reporter - NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The Gli Reporter - NIH3T3 Cell Line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.
Format: aqueous solution containing DMSO
Host Cell Line: NIH/3T3
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Kinzler KW et al. (1990) Mol Cell Biol. 10(2):634-642.
2. Mullor JL et al. (2002) Trends Cell Biol. 12(12):562-569.
3. Peukert S et al. (2010) ChemMedChem. 5(4):500-512.
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