Application Note: Anti-Glial Fibrillary Acidic Protein (Rabbit) antibody has been tested by western blot, ICC, and IHC and is suitable for use in IF. Anti-Glial Fibrillary Acidic Protein antibodies are specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: Sufficient for 10 mini blots (total final volume 100 ml at 1:1000 dilution)
IF Microscopy Dilution: 1:1000
Western Blot Dilution: 1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-GFAP Antibody was produced in rabbit by repeated immunizations with recombinant and purified bovine glial fibrillary acidic protein.
Physical State: Liquid
Purity and Specificity: Anti-GFAP antibody was prepared from monospecifc neat serum. The antibody is directed against bovine glial fibrillary acidic proteins. Expect reactivity with the following species based on sequence homology: human, mouse, rat. Cross reactivity with GFAP from other species has not been determined.
Background: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers. Therefore, GFAP antibody is ideal for investigators involved in neuropathologic diseases and more generally in Neuroscience.
Low Endotoxin: No
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