Clone: Polyclonal
Background: Tested in immunoelectrophoresis, using different antigen/antibody concentration ratio’s the antiserum gives one characteristic precipitin line. In enzyme-immunocytochemical and immunohistochemical staining of concanavalin A in appropriately prepared substrates.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. The entire test system must be free of sodium azide, since it inhibits the enzyme activity.
Source: The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain alpha-D-mannopyranosyl, alpha-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to concanavalin A from jack bean
Formulation: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
UniProt: P02866
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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