Products from BPS Bioscience require a minimum order value above 400€Applications: Generate stable cell line expressing human GPC3 with puromycin selection
Background: GPC3 (Glypican-3, also known as OCI5) is a cell surface proteoglycan highly expressed in the lungs, liver, and kidneys. Its function is tissue-dependent and can either promote or suppress tumorigenesis. Being a heparin sulfate proteoglycan, it is overexpressed in neoplasms including malignant melanoma, hepatocellular carcinoma, and testicular yolk sac tumors in which it regulates cell growth and differentiation. It is also involved in the negative regulation of the hedgehog signaling pathway and in the positive regulation of the non-canonical Wnt signaling pathway.
Description: The GPC3 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human GPC3 (Glypican 3, NM_ 004484.2) driven by an EF1A promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the GPC3 Lentivirus.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: P51654
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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