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Applications: 1. Human GPRC5D binding assays; screen for GPRC5D ligands or antibodies.
2. Target cells for anti-GPRC5D CAR-T development.
Background: The orphan G protein-coupled receptor, class C group 5 member D (GPRC5D) is a retinoic acid-inducible protein with seven transmembrane segments and a short N-terminal extracellular region. The strong and selective expression of GPRC5D in Multiple Myeloma cells, similar to the expression pattern of B-cell maturation antigen (BCMA), makes it a promising target for the treatment of Multiple Myeloma. It is anticipated that GPRC5D will be an alternative CAR T cell target for Multiple Myeloma immunotherapy.
Description: Recombinant HEK293 cells constitutively expressing human GPRC5D (the orphan G protein-coupled receptor, class C group 5 member D, NM_018654.1) under the control of a CMV promoter.
Formulation: For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance. Thaw Medium 1 ( BPS Bioscience #60187):
MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.Growth Medium 1C ( BPS Bioscience #79532):
MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 50 µg/ml of Hygromycin B
Genbank: NM_018654.1
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Uniprot: Q9NZD1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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