Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GRK6 with 0.25 mg/ml PLKtide(substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Background: GRK6 is a Ser/Thr protein kinase and a member of the G protein-coupled receptor kinase subfamily. GRK6 is present in all tissues with strongest expression in placenta and skeletal muscle. GRK6 can phosphorylate proteins such as rhodopsin, ADRB2 and the leukotriene B4 receptor BLT1 (1). GRK6 phosphorylates G protein-coupled dopamine receptors, thereby regulating their activity and mediating desensitization of the receptors. GRK6 knockout mice showed supersensitivity to the locomotor-stimulating effects of psychostimulants, including cocaine and amphetamine (2).
Description: Human GRK6, also known as GPRK6 or FLJ3213 (GenBank Accession No. BC_017272), full length, with N-terminal GST tag, MW ~93 kDa, expressed in Sf9 insect cells via abaculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Genbank: BC017272
Host Cell Line: Sf9 cells
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P43250
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gaudreau, R. et al: Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization. J. Biol. Chem. 277: 31567-31576, 2002.
2. Gainetdinov, R R. et al: Dopaminergic supersensitivity in G protein-coupled receptor kinase 6-deficient mice. Neuron 38: 291-303, 2003.
English
Deutsch
