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Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell poolsOptimized for Use With: Cas9-Expressing Neuro2A Cell Pool (BPS Bioscience, #78087)
Formulation: Growth Medium 1Q (BPS Bioscience #78096): Thaw Medium 1 (BPS Bioscience, #60187) plus 3 µg/ml of Puromycin (InvivoGen, #ant-pr-1).
oThaw Medium 1 (BPS Bioscience #60187): MEM medium (Gibco, #11095-080) supplemented with 10% FBS (Gibco, #26140-079), 1X MEM Non-essential Amino Acids (Corning, #25-025-Cl), 1% Sodium Pyruvate (Corning, #25-000-Cl), and 1% Penicillin/Streptomycin (Gibco, #15140-122).
Mycoplasma Testing: Sterile filtered (0.2 µm) and Mycoplasma-free. Each lot is tested for growth of recombinant cells. All components are low endotoxin. FBS is ISIA-certified and sourced from the United States of America.
Storage Stability: Upon receipt, the medium should be stored in the dark at 4°C. Avoid repeated freeze/thaw cycles. Do not exceed 37°C. When stored in the dark at 4°C, the product is stable for six months after first thaw.
Supplied As: Liquid
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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