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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 2 µM substrate (Ser/Thr 9, Z-Lyte™ Kinase Assay Kit, Invitrogen #PV3324), and GSK3alpha at 37°C for 1 hour.
Description: Human GSK3α (alpha) GenBank Accession No. BC027984), full length, with N-terminal GST-tag, MW=81.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol
Genbank: BC027984
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P49840
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meijer, L. et al., Trends Pharmacol Sci. 25 (9), 471-480 (2004).
2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).
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