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Encompassing Amino Acids: 2-483(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to the GSK3α Assay Kit (BPS Bioscience, #79698) with GSK3a titrated from 200 ng/rxn- 0 ng/rxn.
Description: Human glycogen synthase kinase 3 alpha (GSK3α), also known as GSK3a and GSK3alpha, GenBank Accession No. NM_019884, a.a. 2-483(end), with N-terminal GST-tag and C-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=78 kDa.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl,0.04% Tween20, 3 mM DTT, and 20% glycerol.
Genbank: NM_019884
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: P49840
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meijer, L. et al., Trends Pharmacol Sci. 25 (9), 471-480 (2004).
2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).
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