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Encompassing Amino Acids: 21-32 of NS4A, linker (3 a.a.) and a.a. 31-181 of NS3
Assay Conditions: Assay was performedusing 100 µl HCV assay buffer containing50 mM Tris, pH 7.4, 150 mM NaCl, 5 mMDTT, 10% glycerol, and 5 µM fluorescentlylabeled HCV substrate peptide. Reaction was continuouslymonitored at room temperature for 20 min. at ex350/em500.
Description: Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (3 a.a.) and a.a. 31-181 of NS3 (GenBank Accession No. AF054247) with N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 0.2% Triton X-100, and 10 mM BME.
Genbank: AF054247
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-His-tags
Uniprot: O92972
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lin, C., et al., Hepatitis C Viruse. Norfolk (UK): Horizon Bioscience; 2006. Chapter 6.
2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678.
3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692- 697.
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