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Encompassing Amino Acids: 21-32 of NS4A, linker (3 a.a.) and a.a. 31-181 of NS3
Assay Conditions: Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min at ex350/em500.
Description: Hepatitis C virus (HCV) genotype 2a, also known as HCV2a, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. FJ393024) with N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.2% Triton X-100, 10 mM β-mercaptoethanol, 100 µg/ml FLAG peptide, and 30% glycerol
Genbank: FJ393024
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-His-tags
Uniprot: B8XJX9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jensen, T.B., et al., J. Infect. Dis. 2008;198(12):1756-1765.
2. Wang, Y., et al., Cell Immunol. 2006 May;241(1):38-46.
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