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Encompassing Amino Acids: 1-482 (full length)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20µM BPS HDAC substrate 3 (Catalog #50037), and Mouse HDAC1 (0.3 - 20 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
Description: Mouse HDAC1, GenBank Accession No. NM_008228, amino acids 1-482 (full length), with C-terminal His-tag and C-terminal FLAG-tag, MW = 57 kDa, expressed in an Sf9 baculovirus expression system. Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 100ug/ml flag peptide
Genbank: NM_004964
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: C-terminal His-tag, C-terminal FLAG-tag,
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007).2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).
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