Products from BPS Bioscience require a minimum order value above 400€Applications: Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC3 for drug discovery and HTS applications.
Background: HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules areattached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction ismixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The Fluorogenic HDAC3 Assay Kit is a complete assay systemdesigned to measure histone deacetylase 3 (HDAC3) activity for screening and profilingapplications. It comes in a convenient 96-well format, with all the reagents necessary for100 fluorescent HDAC3 activity measurements. In addition, the kit includes purifiedHDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive andnegative control. The Fluorogenic HDAC3 Assay Kit is based on a unique fluorogenicsubstrate and developer combination. This assay method eliminates dealing with theradioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,only two simple steps on a microtiter plate are needed to analyze the HDAC activitylevel. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate sosubsequent treatment with the Lysine Developer produces a fluorophore that can thenbe measured using a fluorescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Uniprot: O15379
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jia, H., et al., Neurobiology of Disease. 2012 May; 46(2): 351-361
2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar; 6(3): 238-243.Application Reference(s):
Preclinical anti-arthritic study and pharmacokinetic properties of a potent histone deacetylase inhibitor MPT0G009 (2014)
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