Products from BPS Bioscience require a minimum order value above 400€Applications: Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC8 for drug discovery and HTS applications.
Background: HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group fromacetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules areattached to a peptide containing acetyllysine. Attachment to the peptide quenches thefluorescence of the dye. After treatment of the peptide with an HDAC, the reaction ismixed with a development solution that is specific for nonacetylated lysines. If the acetylgroup has been removed from the lysine by the HDAC, this solution will release the dyeallowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The Fluorogenic HDAC 8 Assay Kit is a complete assay systemdesigned to measure histone deacetylase (HDAC) 8 activity for screening and profilingapplications. It comes in a convenient 96-well format, with all the reagents necessary for100 fluorescent HDAC activity measurements. In addition, the kit includes purifiedHDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negativecontrol. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrateand developer combination. This assay method eliminates dealing with the radioactivity,extraction, and chromatography aspects of traditional assays. Using this kit, only twoasimple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First,the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. Thedeacetylation sensitizes the substrate so subsequent treatment with the LysineDeveloper produces a fluorophore that can then be measured using a fluorescencereader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Uniprot: Q9BY41
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Vijayakumar, B., et al. Bioinformation 2011; 7(3): 134-41.
2. Oehme, I., et al. Expert Opin Investig Drugs . 2009 Nov; 18(11): 1605-17.Application Reference(s):
1.
Discovery of inhibitors of Schistosoma mansoni HDAC8 by combining homology modeling, virtual screening, and in vitro validation (2014)
2. Preclinical anti-arthritic study and pharmacokinetic properties of a potent histone deacetylase inhibitor MPT0G009 (2014)
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