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Applications: Screen therapeutic antibodies and ADCs (antibody-drug conjugates) targeting HER3.Co-culture assays with HER3-directed CAR-T cells.
Background: HER3 (human epidermal growth factor receptor 3) is a transmembrane protein encoded by the ERB3 gene. HER3 is broadly expressed in human tissues however, increased expression of HER3 has been linked to a variety of solid tumors including ovarian, breast, colon, and gastric cancers and correlates with decreased overall survival in colorectal cancer patients. HER3 has the unique property of being inherently catalytically inactive but still being able to participate in ligand binding, forming heterodimers with nearby receptors of the HER family. The formation of these dimers initiates a cascade of downstream signaling steps critical to cell proliferation. The binding and subsequent phosphorylation of HER3 by HER2 or EGFR (epidermal growth factor receptor) has been found to play a role in tumor growth and drug resistance. Due to its cell surface expression in a variety of cancers and correlation with decreased survival, HER3 is a prospective therapeutic target for antibody-drug conjugate (ADC) development.
Description: HER3 CHO Cell Line is a CHO-K1 cell line expressing HER3 (human epidermal growth factor receptor 3) (NM_001982.2, also known as receptor tyrosine-protein kinase erbB-3) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lipid-mediated transfection followed by hygromycin selection and limited dilution. Individual clones were screened for HER3 expression levels by flow cytometry, and a clone was selected to generate this cell line.
Host Cell Line: CHO-K1
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: P21860
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
References: Beji A., et al., 2012 Clin Cancer Res. 18(4):956-68
Criscitiello C., et al., 2021 J Hematol Oncol. 14(1):20
Jaiswal B, et al. 2013 Cancer Cell. 23(5):603-17
Littlefield P, et al., 2014 Sci Signal. 7(354):ra114
Zhou B, et al., 2006 Cancer Cell. 10(1):39-50
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