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Encompassing Amino Acids: 1-328
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: HGK is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK (1). The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-α signaling. HGK-dependent signaling inhibits PPARγ-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport (2).
Description: Recombinant human HGK (a.a. 1-328), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NP_004825
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O95819
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yao, Z. et.al: A novel human STE20-related protein kinase, HGK, that specifically activates the c-jun N-terminal kinase signaling pathway. J. Biol. Chem. 274: 2118-2125, 1999.
2. Tang, X. et.al: An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPAR-gamma, adipogenesis, and insulin-responsive hexose transport. Proc. Nat. Acad. Sci. 103: 2087-2092, 2006.
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