Target: HIF 1 alpha
Conjugate: Biotin
Product Type: Monoclonal
Clone Number: ESEE122
Immunogen: Mouse recombinant fragment corresponding to amino acids 329-538
Swiss-Prot: Q61221
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~116kDa. Specific for HIF1Alpha.
Cellular Localization: Cytoplasm,Nucleus
Scientific Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
References: 1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48.2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4.3. Jaakkola P., et al. (2001) Science 292: 468-72.4. Maxwell P.H., et al. (1999) Nature 399: 271-5.5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11.6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9.7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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