Application Note: WB: 1-3μg/ml. IHC-P: 1-2μg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 22
Positive Control: The peptide used to generate this antibody is available for purchase (GTX89722-PEP).
Specificity/Sensitivity: This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
Form: Liquid
Buffer (with preservative): TBS, 0.5% BSA, 0.02% Sodium azide.
Concentration: 0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Background: The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot ID: P61086
Antigen Species: Human
Immunogen: Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1; NP_001104582.1; NP_001104583.1.
Purification: Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation: Unconjugated
Full Name: ubiquitin conjugating enzyme E2 K