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Encompassing Amino Acids: 2-136(end)
Applications: Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.
Assay Conditions: Histone H3.1 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated Histone H3.1 was detected using Chemiluminescence assay.
Description: Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with N-terminal His-tag MW = 15.4 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol
Genbank: NM_003532
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P68431
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Loyola, A., et al., Mol. Cell. 24(2), 309-316 (2006).
2. Hans, F. and Dimitrov, S., Oncogene. 20(24), 3021-3027 (2001). Product Specific Reference(s): 1. Morita, S., et al., Peptides. 48, 75-82 (2013).
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