Products from BPS Bioscience require a minimum order value above 400€Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Histone H3(K9) methyltransferases are enzymes that catalyzethe transfer of a methyl group from the cofactor S-adenosylmethionine to lysine 9residue of histone H3. This group of enzymes includes G9a, SUV39H1, and SUV39H2.
Description: The Histone H3(K9) Universal Methyltransferase Assay Kit is designed for the detection of histone H3(K9) methyltransferase (HMT) activity in screening and profiling applications. The key to theHistone H3(K9) Universal Methyltransferase Assay Kit is a highly specific antibody thatrecognizes methylated K9 residue of Histone H3. With this kit, only three simple steps ona microtiter plate are required for methyltransferase detection. First, Sadenosylmethionineis incubated with a sample containing assay buffer and methyltransferase enzyme for onehour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeledsecondary antibody followed by addition of the HRP substrate to produce chemiluminescencethat can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, purified G9a, SUV39H1 and SUV39H2, and reagents for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.Application Reference(s):
DNA methyltransferase 3a regulates osteoclast differentiation by coupling to an S-adenosylmethionine-producing metabolic pathway (2015)
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